Browsing by Author "Cirit, Umut"

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The effects of a low dose of cabergoline on induction of estrus and pregnancy rates in anestrous bitches
(Elsevier, 2006-09-05) Cirit, Umut; Bacınoğlu, Süleyman; Kaya, Huriye Horoz; Taş, Muzaffer; Ak, Kemal; Cangül, İ. Taci; Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; AAB-4360-2021; 0000-0003-0537-2113; 6602486125
This is the first report of successful induction of normal estrus and ovulation in breeder bitches with as a low dose as 0.6 mu g/kg/day of cabergoline formulation marketed for use in women. Sixty-one pure breed bitches from various breeds were used in the study at their already determined periods of anestrus. Twenty-four dogs formed the control group, while 37 bitches were administered with two different doses of cabergoline (recommended dose group, n = 10, 5 mu g/kg/day and low dose group, n = 27, 0.6 mu g/kg/day). Induced estrus rates and mean treatment and proestrus durations of dogs in these two dose groups were compared. At the second phase of the study, the effects of 500 IU human chorionic gonadotropin (hCG) administered on days 1 and 3 of estrus induced by the low dose of cabergoline, on the duration of behavioral estrus, ovulation rates, pregnancy rates and the number of offspring were investigated. For this purpose, the dogs with signs of proestrus (22/27) following the treatment in the low dose group were assigned into two subgroups. Five hundred IU of hCG (Pregnyl, Organon, Turkey) was intramuscularly administered to eight of these dogs [low dose (hCG+) group] on days 1between days 8-45 and 4-48 (mean: 23.63 +/- 14.33 and 24.41 +/- 14.31 days), in the ratio of 80.0 and 81.5%, respectively (p > 0.05). In both dose groups, post-treatment interestrous intervals were significantly shorter than both those of the control group and their own pre-treatment interestrous intervals (p < 0.05). Ovulation rates, pregnancy rates and mean number of offspring delivered by the dogs in the recommended dose, low dose (hCG-), low dose (hCG+) and control groups were found to be similar (p > 0.05). However, the mean duration of behavioral estrus of the dogs in the low dose (hCG+) group was found to be significantly longer compared to dogs in all other groups (p < 0.05). In both dose groups, no correlation could be found between the anestrus stages and treatment durations (p > 0.05). Shortly, it has been concluded from the study that (1) normal and fertile estrus can be induced more economically in bitches during different stages of anestrus using as a low dose of 0.6 mu g/kg of cabergoline formulation marketed for use in women, and that (2) hCG injections on days 1 and 3 of the estrus induced by this method has no positive effects on the ovulation rates, pregnancy rates and the number of offspring per pregnancy. (C) 2006 Elsevier B.V. All rights reserved. and 3 of estrus. The remaining 14 dogs were not treated with hCG [low dose (hCG-) group]. An aqueous solution of cabergoline (Dostinex, Pharmacia, Italy) was orally administered until 2 day after the onset of proestrus or for a maximum of 42 days. Blood samples were taken daily from all treatment and 11 control bitches during the first five days of behavioral estrus to measure progesterone concentrations. In the recommended dose and low dose groups, estrus was induced.
Prediction of postpubertal reproductive potential according to prepubertal body weight, testicular size, and testosterone concentration using multiple regression analysis in Kivircik ram lambs
(TÜBİTAK, 2008) Cirit, Umut; Demir, Hıdır; Elmaz, Özkan; Dikmen, Serdal; Uludağ Üniversitesi/Veteriner Fakültesi/Hayvancılık Anabilim Dalı; 0000-0002-5611-4993; A-5731-2018; 6506616870;
The relationship between the prepubertal body weight, testicular size, testosterone concentration, and postpubertal reproductive function was investigated in Kivircik ram lambs. The body weight, testicular size, and testosterone concentration were measured every 20 days between 60 and 420 days of age. Semen was collected from the ram lambs at 7, 8, 9, 10, 11, 12, 13 and 14 months of age. Data obtained were analyzed by best subsets regression model. We determined that body weight, scrotal circumference, testicular length, and testosterone levels in the early prepubertal period (days 80, 100, and 120), when combined with each other, allow the determination of the postpubertal (days 220, 240, 260 and 280) testicular diameter. Body weight and testosterone at prepubertal ages, either alone or in combination with other variables, were related to semen volume at postpubertal ages (at 7, 8, 9, 11, and 14 months of age). It is concluded that there is a significant relation between postpubertal reproduction characteristics and the selection of Kivircik ram lambs during the prepubertal period (especially between days 80 and 120) according to body weight, testicular characteristics, and testosterone hormone concentrations. According to the relationship among these characteristics, selection of ram lambs at juvenile ages will improve the effectiveness of the selection and then the economic gain of sheep-breeding operations
Using cell banks as a tool in conservation programmes of native domestic breeds: the production of the first cloned Anatolian Grey cattle
(Csiro Publishing, 2011) Arat, Sezen; Çaputçu, Arzu Taş; Akkoç, Tolga; Pabuccuoğlu, Serhat; Cirit, Umut; Koban, Evren; Bağış, Haydar; Demir, Kamber; Şenünver, Adem; Kılıçaslan, Ragip; Çetinkaya, Gaye; Denizci, Melis; Aslan, Özgür; Sağırkaya, Hakan; Nak, Yavuz; Nak, Deniz; Tuna, Bilginer; Uludağ Üniversitesi/Veterinerlik Fakültesi/Üreme ve Suni Tohumlama Bölümü.; Uludağ Üniversitesi/Veterinerlik Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; AAH-5494-2021; 6602400461; 8615464000; 9280090000
The aim of this study was to clone native Anatolian Grey cattle by using different donor cell types, such as fibroblast, cartilage and granulosa cells cryopreserved in a gene bank and oocytes aspirated from ovaries of Holstein cows as the recipient cytoplasm source. One male calf from fibroblast, three female calves from granulosa cells and one female calf from cartilage cells were born healthy and at normal birthweights. No calves were lost after birth. The results demonstrated that the cloned calves had the same microsatellite alleles at 11 loci as their nuclear donors. However, the mtDNAs of the five Anatolian Grey cloned calves had different haplotypes from their donor cells and mtDNA heteroplasmy could not be detected in any of the clones. The birth of healthy clones suggests that the haplotype difference between the cell and oocyte donor did not affect the pre- or post-implantation development of the bovine nuclear transfer derived embryos in our study. The results showed that well established nuclear transfer protocols could be useful in conserving endangered species. In conclusion, somatic cell banking can be suggested as a tool in conservation programmes of animal genetic resources.