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Title: Rapid identification of avian Salmonella isolates by air thermal cycler amplification and capillary gel electrophoresis
Other Titles: Kanatlı Salmonella izotlarının air thermal cycler amplifikasyonu ve kapiller jel elektroforezi ile hızlı identifikasyonu
Authors: Uludağ Üniversitesi/Veteriner Fakültesi/Besin Hijyeni ve Teknolojisi Bölümü.
Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Bölümü.
Çarlı, Kamil Tayfun
Caner, Vildan
Eyigör, Ayşegül
Keywords: Polymerase chain reaction
Enteritidis infections
Issue Date: 2001
Publisher: TÜBİTAK
Citation: Çarlı, K. T. vd. (2001). "Rapid identification of avian Salmonella isolates by air thermal cycler amplification and capillary gel electrophoresis". Turkish Journal of Veterinary & Animal Science, 25(5), 717-720.
Abstract: The possible use of a genus-specific polymerase chain reaction (PCR) for the identification of Salmonella colonies was tested on 14 reference strains and 38 clinical Salmonella strains isolated from chickens by the standard method of the National Poultry Improvement Plan and Auxiliary Provisions (NPIP), USDA, U.S.A. All the PCRs using primer pairs InvA1 and InvA2 (5) gave 281 bp amplification product specific for the Salmonella genus and produced results identical (100%) to those obtained with biochemical and serological methods. Thus, the genus-specific capillary PCR for the identification of a colony of Salmonellae from a selective agar plate was performed within approximately 2 h and the total time required for definitive diagnosis of infection was 2 days using primary enrichment (PE) and 7 days using delayed secondary enrichment (DSE).
ISSN: 1300-0128
Appears in Collections:Scopus
Web of Science

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