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Title: A glance at the methods for detection of apoptosis qualitatively and quantitatively
Other Titles: Apoptozun nitel ve nicel olarak tespitine yönelik metotlara kısa bir bakış
Authors: Ulukaya, Engin
Açılan, Ceyda
İlkay Armutak, Elif
Yılmaz, Yusuf
Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Moleküler Biyoloji Anabilim Dalı.
Arı, Ferda
Keywords: Biochemistry & molecular biology
Hematoxylin/giemsa staining
Propidium Iodide/hoechst staining
Annexin V-FITC
M30 Antigen
DNA fragmentation
Cytochrome c
Caspase-cleaved cytokeratin-18
Neoadjuvant chemotherapy
Issue Date: 2011
Publisher: Walter De Gruyter
Citation: Ulukaya, E. vd. (2011). "A glance at the methods for detection of apoptosis qualitatively and quantitatively". Turkish Journal of Biochemistry, 36(3), 261-269.
Abstract: While programmed cell death, apoptosis, occurs as a necessary and natural event for multicellular organisms, necrosis is a form of unplanned cell death as a result of pathological or chemical trauma. There are numerous molecular and morphological differences between these two forms of cell death, whose decision is based on the type and dose of the stress. As apoptosis is critical for homeostasis of an organism, i.e. for development to adult stage, disease progression, or response to different stimuli, it is being studied more extensively in the area of basic research and clinics, and the need for quick detection of apoptosis and well established criteria for the discrimination of apoptosis have also gained more popularity. Here, we review our knowledge on the most commonly used methods for both qualitatively and quantitatively measuring apoptosis, including morphological imaging (i.e. through light, fluorescence, phase contrast or electron microscopy), immunohistochemical (i.e. Annexin V-FITC, TUNEL, M30 antigen or caspase 3 detection), biochemical (i.e. DNA-or protein-based electrophoresis or flow cytometry-based methods), immunological (i.e. ELISA), and molecular biology techniques (i.e. array-based techniques) while focusing on the differences for distinction between the two forms of cell death. Indeed, one has to confirm that cell death occurs through apoptosis based on more than one of these protocols depending on the specific purpose of the user.
ISSN: 0250-4685
Appears in Collections:Scopus
Web of Science

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