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Title: Detection of shiga-toxin producing e.coli (stec), enteropathogenic e.coli (epec) and enterotoxigenic e.coli (etec) from animals by m-pcr
Authors: Khider, Mohammed
Bursa Uludağ Üniversitesi/Veteriner Fakültesi.
Bursa Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü.
Büyükcangaz, Esra
Sen, Aysin
Cengiz, Murat
Sonal, Songül
Keywords: Antimicrobial susceptibility
E. coli
Issue Date: 10-Nov-2019
Publisher: Bursa Uludağ Üniversitesi
Citation: Büyükcangaz, E. vd. (2019). "Detection of shiga-toxin producing e.coli (stec), enteropathogenic e.coli (epec) and enterotoxigenic e.coli (etec) from animals by m-pcr". Bursa Uludağ Üniversitesi Veteriner Fakültesi, 38(2), 88-94.
Abstract: This research investigated the presence of virulence genes encoding F41, K99, eae, Stx1, Stx2 and STa and the antimicrobial resistance of animal Escherichia coli (E. coli) isolates. Clinical isolates (n:233) were evaluated from fecal samples of cattle, sheep, goats, horses, cats and dogs collected between the years of 2010 to 2015 from Turkey. Enterohaemorrahic E.coli (EHEC) O157:H7 was detected by using cefixime tellurite sorbitol MacConkey agar (CT-SMAC) and Wellcollex E. coli (Remel®). The Kirby-Bauer disc diffusion test was performed to detect the resistance pattern of the isolates to ampicillin, Amoxycilin/clavulanic acid, enrofloxacin, ceftiofur, trimethoprim/sulfamethoxazole and tetracycline. The results showed that 40% of the ruminant isolates were identified as Shiga-toxin producing Escherichia coli (STEC). Enterotoxigenic E. coli (ETEC) was detected in samples from cattle (0.9%) and sheep (12%). Enteropathogenic E.coli (EPEC) was detected in samples from cattle (0.9%) and dogs (11.4%). EHEC O157:H7 was not detected any of the isolate. Among all E.coli isolates that carried at least one virulence gene, 8 (19%) were resistant to more than three antimicrobials, 7 (16.7%) were resistant to at least one antimicrobial and 27 (64.3%) were susceptible to all antimicrobials.
ISSN: 1301-3173
Appears in Collections:2019 Cilt 38 Sayı 2

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