Yenilebilir yabani bitki Gundelia tournefortii’nin antioksidan özelliklerinin belirlenmesi
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Date
2017-06-14
Authors
Konak, Merve
Journal Title
Journal ISSN
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Publisher
Uludağ Üniversitesi
Abstract
Yabani yenebilir bitkiler tüm dünyada besinsel ve fonksiyonel özelliklerinden dolayı yaygın olarak kullanılmaktadır. Türkiye bitki biyoçeşitliliği bakımından en zengin ülkelerden biridir. Türkiye’de birçok yabani bitki geleneksel olarak halk tarafından tüketilmektedir. Asteraceae familyasına ait olan Gundelia tournefortii, Doğu Anadolu’dan Batı Asya’ya kadar bilinmektedir. Bu bitki ülkemizde kenger otu, kenger sakızı, sakız otu, çadır dikeni, kanak sakızı gibi çeşitli isimlerle de anılmaktadır. Yaprakları ve sapları gıda olarak kullanılmakta özellikle salata ve çorbalara katılmaktadır. Ayrıca, Gundelia tournefortii orta doğuda, geleneksel tıpta karaciğer koruyucu, diyabet karşıtı, ağrı kesici ve sindirime yardımcı olarak da kullanılmaktadır. Bu çalışmanın amacı, Diyarbakır bölgesinden toplanan Gundelia tournefortii’in toplam fenol içeriği ve antioksidan kapasitesinin belirlenmesi olup ayrıca bunların biyoalınabilirliklerinin de incelenmesidir. Toplam fenol içeriğinin belirlenmesi için Folin-Ciocalteu metodu, antioksidan kapasite tayini için ise CUPRAC, ABTS ve DPPH metotları kullanılmıştır. Biyoalınabilirliğin saptanması için sindirim sistemi taklit edilerek ortam koşulları in-vitro olarak sağlanmış ve bitki örnekleri bu ortamda enzimatik ekstraksiyon işlemine tabi tutulmuştur. Bitkinin sap kısımlarının ekstrakte edilebilir toplam fenolik içeriği 700,21 mg GAE /100g iken, hidrolize edilebilir toplam fenolik içeriği, 1119,23 mg GAE /100g bulunmuştur. Antioksidanların biyoalınabilirliği ABTS metoduyla yaklaşık olarak %95 olarak belirlenmiştir. Sonuç olarak, bu çalışma Gundelia tournefortii bitkisinin potansiyel bir antioksidan kaynağı olduğunu ve doğal bir antioksidan kaynağı olarak günlük olarak tüketilebileceğini göstermektedir.
Wild edible plants have been commonly used as food sources all over the world, because of their nutritional and functional properties. Turkey is one of the richest countries of the world in terms of plant diversity. Several wild edible plants are traditionally used in nutrition and consumed as vegetable in Turkey. Gundelia tournefortii L. belongs to the Asteraceae (Compositae) family, is a well-known plant in Eastern Anatolia to Western Asia. This plant locally known as “Kenger otu, kenger sakızı, sakız otu, çadır dikeni, kanak sakızı” in Turkey. Its leaves and stems are used as food especially ingredients in soups and salads. In addition, Gundelia tournefortii has been commonly used in traditional medicine as a hepatoprotective, a potential remedy for diabetes, chest pain and digestive in the Middle East. The main objectives of this study were to determine the total phenolic contents and antioxidant capacities of Gundelia tournefortii and to examine the phenolic bioaccessibilities of this plant collected from Diyarbakır region. Folin-Ciocalteu method was used for total phenolic content. Antioxidant capacity assay was stated according to CUPRAC, ABTS and DPPH methods. For the determination of bioaccessibility, plant samples were processed by an in-vitro digestive enzymatic extraction that mimics the conditions in the gastrointestinal tract. The extractable total phenolic content of Gundelia tournefortii L. stems were 700,21 mg GAE /100g fw, whereas hydrolyable phenolics were 1119,23 mg GAE /100g fw, respectively. Bioaccessibility of antioxidants were determined average 95% by ABTS method. The study indicated that Gundelia tournefortii have the potential to be sources of antioxidant components and can be included in the daily diet as a natural antioxidant.
Wild edible plants have been commonly used as food sources all over the world, because of their nutritional and functional properties. Turkey is one of the richest countries of the world in terms of plant diversity. Several wild edible plants are traditionally used in nutrition and consumed as vegetable in Turkey. Gundelia tournefortii L. belongs to the Asteraceae (Compositae) family, is a well-known plant in Eastern Anatolia to Western Asia. This plant locally known as “Kenger otu, kenger sakızı, sakız otu, çadır dikeni, kanak sakızı” in Turkey. Its leaves and stems are used as food especially ingredients in soups and salads. In addition, Gundelia tournefortii has been commonly used in traditional medicine as a hepatoprotective, a potential remedy for diabetes, chest pain and digestive in the Middle East. The main objectives of this study were to determine the total phenolic contents and antioxidant capacities of Gundelia tournefortii and to examine the phenolic bioaccessibilities of this plant collected from Diyarbakır region. Folin-Ciocalteu method was used for total phenolic content. Antioxidant capacity assay was stated according to CUPRAC, ABTS and DPPH methods. For the determination of bioaccessibility, plant samples were processed by an in-vitro digestive enzymatic extraction that mimics the conditions in the gastrointestinal tract. The extractable total phenolic content of Gundelia tournefortii L. stems were 700,21 mg GAE /100g fw, whereas hydrolyable phenolics were 1119,23 mg GAE /100g fw, respectively. Bioaccessibility of antioxidants were determined average 95% by ABTS method. The study indicated that Gundelia tournefortii have the potential to be sources of antioxidant components and can be included in the daily diet as a natural antioxidant.
Description
Keywords
Antioksidan kapasite, Biyoalınabilirlik, Toplam fenol, CUPRAC, DPPH, ABTS, Antioxidant capacity, Bioaccessibility, Total phenolic content
Citation
Konak, M. vd. (2017). "Yenilebilir yabani bitki Gundelia tournefortii’nin antioksidan özelliklerinin belirlenmesi". Uludağ Üniversitesi Ziraat Fakültesi Dergisi, 31(2), 101-108.