Determination of genetic relationship among some walnut (Juglans regia L.) genotypes and their early-bearing progenies using RAPD markers
Date
2011
Authors
Dalkılıç, Zeynel
Journal Title
Journal ISSN
Volume Title
Publisher
Ars Docendi
Abstract
Random amplified polymorphic DNA (RAPD) method was used to study the genetic relationships among eight walnut genotypes grown in Turkey. A total of 45 10-mer primers were tested with the RAPD technique. Thirty-seven primers showed reproducible polymorphic pattern. These primers produced 513 bands, out of which 340 were polymorphic. The molecular size varied between 200 - 5000 bp. The dendrogram realized from the RAPD markers grouped the eight genotypes into three major clusters. The highest similarity was found between NO-2 and NO-8 genotypes (0.779). The cophenetic correlation coefficient between the similarity matrix and the cophenetic matrix of the dendrogram was very high (r = 0.9131), supporting the validity of the dendrogram. Based on these results, RAPD analysis can be used for the characterization and grouping of walnut genotypes.
Description
Keywords
Biotechnology & applied microbiology, Juglans regia L., Genetic diversity, RAPD, Cultivars, Variability, Relatedness, Diversity, Juglans, Juglans regia
Citation
Ertürk, Ü. vd. (2011). "Determination of genetic relationship among some walnut (Juglans regia L.) genotypes and their early-bearing progenies using RAPD markers". Romanian Biotechnological Letters, 16(1), 5944-5952.