Rapid Bielschowsky silver impregnation method using microwave heating
Date
2001
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Taylor & Francis
Abstract
The Bielschowsky silver impregnation method has been used extensively to demonstrate neuronal processes including dendrites, axons and neurofibrils. In this study, we examined the differences in the time required for and the staining quality of the Bielschowsky method for neuronal processes when microwave heating was used instead of processing at room temperature. For this purpose, a control group of sections stained according to the conventional method at room temperature was compared to an experimental group stained in a microwave oven at 180 W for 2, 4 and 1 min in 2% silver nitrate, ammoniacal silver nitrate and gold chloride, respectively. Light microscopic examination demonstrated that the normal structure was preserved in both groups and that there was no difference in the staining quality between the control and the microwave groups. In addition, staining time for this procedure was reduced to 8 min by using the microwave oven. Our study revealed that microwave irradiation can be used safely for Bielschowsky silver impregnation of neuronal tissues.
Description
Keywords
Biotechnology & applied Microbiology, Microwave irradiation, Cell biology, Neural tissue, Silver impregnation, Histopathology, Sections, Fixation
Citation
Minbay, F. Z. vd. (2001). "Rapid Bielschowsky silver impregnation method using microwave heating". Biotechnic & Histochemistry, 76(5-6), 233-237.