Changes in peroxidase activities and soluble proteins in strawberry varieties under salt-stress
Date
2006
Authors
Turhan, Ece
Journal Title
Journal ISSN
Volume Title
Publisher
Springer Heidelberg
Abstract
Effects of salt stress on the activity of peroxidase (PRX) isozyme and leaf proteins were studied in the three strawberry varieties, Camarosa, Tioga and Chandler. Plants were watered with modified 1/3 Hoagland nutrient solution containing 0 (control), 8.5, 17.0 and 34.0 mM NaCl for 30 days following 20 days acclimation. PRX activity was assayed in leaf extracts and PRX profiles of control and salt treated plants were performed by native polyacrylamide gel electrophoresis (PAGE). During the salt stress Tioga and Camarosa tolerated the cellular damage with less electrolyte leakage while Chandler exhibited a severe cellular damage with the highest (87.5% in 34.0 mM NaCl treatment) electrolyte leakage. Total soluble protein content was decreased by salinity in Tioga (from 11.35 to 5.86 mg center dot g(-1) DW) and Chandler (from 9.35 to 3.90 mg center dot g(-1) DW) while it was almost unchanged in Camarosa. Salt stress increased total and specific PRX activity as compared with the control in all the varieties. In native PAGE, one basic isoperoxidase band (Rf=0.27) was observed commonly with different band intensity in all the treatments. Therefore, it might be associated with lignification and recovery of cell membrane damage in strawberry plants under salt stress.
Description
Keywords
Plant sciences, Strawberry, Salt stress, Protein, Peroxidase, Biosynthesis, Electrophoresis, Plants, Salinity, Growth, Tolerance, Cold-acclimation, Osmotic adjustment, Superoxide-dismutase, Persical batsch, Fragaria x ananassa
Citation
Gülen, H. vd. (2006). ''Changes in peroxidase activities and soluble proteins in strawberry varieties under salt-stress''. Acta Physiologiae Plantarum, 28(2), 109-116.