2006 Cilt 6 Sayı 3
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Item Open Access Control of American foulbrood disease without antibiotic use(Uludağ Üniversitesi, 2006) Parvanov, P.; Russenov, N.; Dimov, D.With regard to the prohibited use of veterinary preparations containing antibiotics and sulfonamides in apicultural practice in the Republic of Bulgaria, attempts for healing apiaries affected by American Foulbrood without recourse to these drugs were performed. Three apiaries with a total of 119 bee families were healed. The healing of bee colonies was achieved via quarantine, destruction of affected bee families via burning and disinfection of hives with 4% sodium hydroxide. Following two negative examination at 15-day intervals for clinical signs of diseased bee families, a microbiological examination of bee honey, sampled from all families in the apiaries, for contamination with Paenibacillus larvae ssp. larvae spores was performed. The bee families, contaminated with spores, were moved as an “artificial swarm” in disinfected hives on new frames with wax foundation. Prior to moving, the bees were kept for 48 hours without food in a cool premise. In bee families, diagnosed as diseased at the end of the active season, the examination of honey for contamination with spores and the transfer of swarms were done in the spring of the next year. The old combs of these families were melted and the nests were broadened with new frames for building combs. The apiaries were considered as healed and the quarantine was removed one year after the last destruction of a bee colony and negative results in the examination of honey samples for contamination with spores.