Benzofuran kalkon türevi kimyasalların insan akciğer kanseri hücreleri üzerindeki sitotoksik etkikerinin araştırılması
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Date
2020-09-21
Authors
Avcı, Hediye
Journal Title
Journal ISSN
Volume Title
Publisher
Bursa Uludağ Üniversitesi
Abstract
Akciğer kanseri dünyada ve ülkemizde en sık rastlanan sağlık sorunlarından biridir. Günümüzde akciğer kanseri için çeşitli tedavi yöntemleri (cerrahi operasyon, kemoterapi, radyoterapi) geliştirilmiş olmasına rağmen yeni stratejilere ve ilaçlara ihtiyaç duyulmaktadır. Özellikle doğal kaynaklardan elde edilebilecek anti kanser ajanlar oldukça ilgi çekmektedir. Anti kanser aktiviteye sahip olan flavonoidlerin bir alt grubu olan kalkonlar son yıllarda biyolojik aktiviteleri bakımından büyük bir ilgi görmektedir. Farklı yapılardaki doğal ya da sentetik kalkon türevlerinin kanser hücrelerinde anti kanser aktiviteye sahip olduğu gözlenmiştir. Bu tez çalınmasında sentezi ve karakterizasyonları gerçekleştirilen benzofuran sübstitüe kalkon türevlerinin (Kompleks 1 ve Kompleks 2) antikanser aktivitesi insan akciğer kanser hücreleri (A549 ve H1299) üzerinde arattırılmıştır. Komplekslerin, sağlıklı hücrelerde olası etkilerini belirleyebilmek için sağlıklı akciğer hücre soyu (BEAS-2B) kullanılmıştır. Komplekslerin hücre canlılığı üzerindeki sitotoksik etkisi SRB (24, 48 ve 72 saat) ve ATP (48 ve 72 saat) canlılık testleri ile belirlenirken, hücrelerde ki ölüm modu (apoptosis/nekrozis), floresan boyama ile belirlenmiştir. Ayrıca komplekslerin hücrelerdeki migrasyon yetenekleri yara iyileşmesi testi ile araştırılmıştır. Sonuç olarak benzofuran sübstitüe kalkon türevlerinin insan akciğer kanser hücrelerinde doza ve zamana bağlı olarak sitotoksik aktivite göstererek hücre büyümesini inhibe ettiği bulunmuştur. Ayrıca bu komplekslerin hücrelerde apoptozu indükleyerek, invazyon yeteneklerinde azalmaya sebep olduğu belirlenmiştir. Alınan sonuçlara göre benzofuran sübstitüe kalkon türevlerinin akciğer kanseri tedavisinde umut vadeden Ajanlar olabileceği ve ileri analizlerin yapılması gerektiği kanaatine varılmıştır.
Lung cancer is one of the most common health problems in the world and in our country. Although various treatment methods (surgical operation, chemotherapy, radiotherapy) have been developed for lung cancer, new strategies and drugs are needed. In particular, anti-cancer agents that can be obtained from natural sources attract great attention. Chalcones, which are a subgroup of flavonoids with anticancer activity, have attracted great interest in terms of their biological activities in recent years. It has been observed that natural or synthetic chalcone derivatives with different structures have anticancer activity in cancer cells. In this thesis, the anticancer activity of benzofuran substituted chalcone derivatives (Complex 1 and Complex 2) whose synthesis and characterization was performed was investigated on human lung cancer cells (A549 and H1299). The healthy lung cell line (BEAS-2B) was used to determine the possible effects of the complexes on healthy cells. Cytotoxic effect of the complexes on cell viability was determined by SRB (24, 48 and 72 hours) and ATP (48 and 72 hours) viability tests, while the mode of death (apoptosis / necrosis) in cells was determined by fluorescent staining. In addition, the migration abilities of the complexes in cells were investigated by the wound healing test. As a result, it was found that benzofuran substituted chalcone derivatives inhibited cell growth in human lung cancer cells by showing cytotoxic activity depending on dose and time. In addition, it has been determined that these complexes induce apoptosis in cells, causing a decrease in invasion capabilities. According to the results, it was concluded that benzofuran substituted chalcone derivatives could be promising agents in the treatment of lung cancer and further analysis should be done.
Lung cancer is one of the most common health problems in the world and in our country. Although various treatment methods (surgical operation, chemotherapy, radiotherapy) have been developed for lung cancer, new strategies and drugs are needed. In particular, anti-cancer agents that can be obtained from natural sources attract great attention. Chalcones, which are a subgroup of flavonoids with anticancer activity, have attracted great interest in terms of their biological activities in recent years. It has been observed that natural or synthetic chalcone derivatives with different structures have anticancer activity in cancer cells. In this thesis, the anticancer activity of benzofuran substituted chalcone derivatives (Complex 1 and Complex 2) whose synthesis and characterization was performed was investigated on human lung cancer cells (A549 and H1299). The healthy lung cell line (BEAS-2B) was used to determine the possible effects of the complexes on healthy cells. Cytotoxic effect of the complexes on cell viability was determined by SRB (24, 48 and 72 hours) and ATP (48 and 72 hours) viability tests, while the mode of death (apoptosis / necrosis) in cells was determined by fluorescent staining. In addition, the migration abilities of the complexes in cells were investigated by the wound healing test. As a result, it was found that benzofuran substituted chalcone derivatives inhibited cell growth in human lung cancer cells by showing cytotoxic activity depending on dose and time. In addition, it has been determined that these complexes induce apoptosis in cells, causing a decrease in invasion capabilities. According to the results, it was concluded that benzofuran substituted chalcone derivatives could be promising agents in the treatment of lung cancer and further analysis should be done.
Description
Keywords
Akciğer kanseri, Apoptoz, Antikanser aktivite, Kalkonlar, Anticancer activity, Chalcones, Apoptosis, Lung cancer
Citation
Avcı, H. (2020). Benzofuran kalkon türevi kimyasalların insan akciğer kanseri hücreleri üzerindeki sitotoksik etkikerinin araştırılması. Yayınlanmamış yüksek lisans tezi. Bursa Uludağ Üniversitesi Fen Bilimler Enstitüsü.