Mildiyöye (Plasmopara halstedii (Farlow) berlese & de toni) dayanıklı bazı yağlık ayçiçeği ebeveyn hatlarının çoklu dizi (Line x tester) analiz yöntemi ile melez performanslarının belirlenmesi
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Date
2020-01-28
Authors
Kızık, Sezgin
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Publisher
Bursa Uludağ Üniversitesi
Abstract
Bu araştırma, mildiyöye dayanıklı yağlık ayçiçeği ebeveyn hatları ve melez kombinasyonlarının verim ve kalite özellikleri üzerinde istatistiksel ve genetik analizler yapılarak performansı yüksek ebeveynleri ve hibrit kombinasyonlarını belirlemek amacıyla 2016-2017 yıllarında Agromar Marmara Tarım Ürünleri A.Ş. firmasının ıslah tarlalarında yürütülmüştür. Araştırmada genetik materyal olarak Agromar A.Ş. tarafından geliştirilen ileri generasyon 5 adet sitoplazmik erkek kısır (CMS) hat ve 4 adet restorer hat kullanılmıştır. Araştırmanın ilk yılında mildiyöye karşı dayanıklılığı belirlenen 5 adet CMS ana hat ve 4 adet restorer baba hat Line x Tester desenine göre melezlenerek 20 adet melez kombinasyonu elde edilmiştir. İkinci yılda 20 adet melez kombinasyonu ve 9 adet ebeveyn hat Tesadüf Blokları Deneme Deseninde 3 tekerrürlü olarak denenmiş ve Line x Tester analiz metoduna göre test edilerek bazı istatistik ve genetik analizler yapılmıştır. Araştırma sonuçlarına göre, kullanılan ebeveyn ve melezlerin tamamının mildiyöye dayanıklı olduğu tespit edilmiştir. Tane verimi ve yağ verimi bakımından CMS2, CMS3 ve Restorer4 hsatları pozitif yönde, CMS5 ve Restorer 1 hatları ise negatif yönde ve önemli genel uyum yeteneği etkisi (GUY) göstermiştir. Özel uyum yeteneği etkileri tane verimi bakımından 7 melez kombinasyonda ve yağ verimi bakımından ise 9 melez kombinasyonda pozitif yönde önemli bulunmuştur. Öte yandan CMS2 x Restorer4, CMS2 x Restorer2 ve CMS3 x Restorer4 melezleri hem verim hem de kalite özellikleri bakımından üstün bulunup ümitvar çeşit adayı olarak belirlenmiştir. CMS1 x Restorer2, CMS3 x Restorer2 ve CMS3 x Restorer1 melezleri yağ oranı bakımından üstün bulunmasına rağmen verim bakımından istenilen düzeye ulaşamamıştır. CMS3 x Restorer3, CMS4 x Restorer4 ve CMS1 x Restorer1 melezleri ise verim yönünden üstün bulunup, yağ oranı açısından istenilen sonucu verememiştir.
This research was conducted in the breeding fields of Agromar Marmara Tarım Ürünleri A.Ş during 2016-2017 years in order to determine parents and hybrid combinations with high performance by performing statistical and genetic analyzes on yield and quality characteristics of mildew resistant oilseed sunflower parental lines and hybrid combinations. Five advanced generation cytoplasmic male sterile (CMS) lines and four restorer lines developed by Agromar A.Ş were used as genetic materials in the study. In the first year of the research, 20 hybrid combinations were obtained by crossing according to Line x Tester design between five cytoplasmic male sterile female lines and four restorer male lines with resistant to mildew. In the second year, 20 hybrid combinations and 9 parent lines were examined in Randomized Blocks Design with 3 replications and some statistical and genetic analyzes were performed according to Line x Tester analysis method. According to the results of the research, it was determined that all the parents and hybrids used were resistant to mildew. In terms of grain yield and oil yield, CMS2, CMS3 and Restorer4 parental lines had positive significant GUY effect, while CMS5 and Restorer 1 parental lines showed negative and significant GUY effects. Specific combining ability (SCA) effects were found to be significant in 7 hybrid combinations in terms of seed yield and 9 hybrid combinations in terms of oil yield. On the other hand, CMS2 x Restorer 4, CMS2 x Restorer2 and CMS3 x Restorer4 hybrids were found to be superior in terms of both yield and quality characteristics and were identified as promising cultivar candidates. Although CMS1 x Restorer2, CMS3 x Restorer2 and CMS3 x Restorer1 hybrids were superior in terms of oil content, they did not reach the desired level in terms of seed yield. The hybrids, CMS3 x Restorer3, CMS4 x Restorer4 and CMS1 x Restorer1 were found to be superior in terms of seed yield but did not yield the desired results in terms of oil content.
This research was conducted in the breeding fields of Agromar Marmara Tarım Ürünleri A.Ş during 2016-2017 years in order to determine parents and hybrid combinations with high performance by performing statistical and genetic analyzes on yield and quality characteristics of mildew resistant oilseed sunflower parental lines and hybrid combinations. Five advanced generation cytoplasmic male sterile (CMS) lines and four restorer lines developed by Agromar A.Ş were used as genetic materials in the study. In the first year of the research, 20 hybrid combinations were obtained by crossing according to Line x Tester design between five cytoplasmic male sterile female lines and four restorer male lines with resistant to mildew. In the second year, 20 hybrid combinations and 9 parent lines were examined in Randomized Blocks Design with 3 replications and some statistical and genetic analyzes were performed according to Line x Tester analysis method. According to the results of the research, it was determined that all the parents and hybrids used were resistant to mildew. In terms of grain yield and oil yield, CMS2, CMS3 and Restorer4 parental lines had positive significant GUY effect, while CMS5 and Restorer 1 parental lines showed negative and significant GUY effects. Specific combining ability (SCA) effects were found to be significant in 7 hybrid combinations in terms of seed yield and 9 hybrid combinations in terms of oil yield. On the other hand, CMS2 x Restorer 4, CMS2 x Restorer2 and CMS3 x Restorer4 hybrids were found to be superior in terms of both yield and quality characteristics and were identified as promising cultivar candidates. Although CMS1 x Restorer2, CMS3 x Restorer2 and CMS3 x Restorer1 hybrids were superior in terms of oil content, they did not reach the desired level in terms of seed yield. The hybrids, CMS3 x Restorer3, CMS4 x Restorer4 and CMS1 x Restorer1 were found to be superior in terms of seed yield but did not yield the desired results in terms of oil content.
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Keywords
Ayçiçeği, Sunflower, Kombinasyon kabiliyeti, Heterosis, Verim ve kalite, Mildiyö, Combining ability, Heterosis, Yield, Quality, Mildew
Citation
Kızık, S. (2020). Mildiyöye (Plasmopara halstedii (Farlow) berlese & de toni) dayanıklı bazı yağlık ayçiçeği ebeveyn hatlarının çoklu dizi (Line x tester) analiz yöntemi ile melez performanslarının belirlenmesi. Yayınlanmamış yüksek lisans tezi. Bursa Uludağ Üniversitesi Fen Bilimleri Enstitüsü.